Compared to Drosophila and humans, the apoptosis network in nematodes is much simpler. Īpoptotic mechanisms are being clarified in model organisms using completed genome sequences, especially in nematodes, fruit flies and humans. There are very important connections between apoptotic cell death and autophagic cell death, as they occur concurrently in many processes. Cell death by autophagy involves cell degradation by internal lysozymes. Apoptosis is characterized by morphological changes and biochemical events such as cytoplasmic and nuclear condensation, phosphatidylserine extrusion, vacuolization, chromatin condensation, DNA fragmentation, and formation of apoptotic bodies that are ultimately engulfed by surrounding cells or macrophages. There are two primary programmed cell death signaling pathways, apoptosis and autophagy, of which apoptosis has been researched more extensively. ![]() Programmed cell death, as well as cell proliferation and cell differentiation, has a crucial role in biological growth and development. These results lay the foundation for further apoptosis-related study in Bombyx mori. We hypothesized that the classic intrinsic and extrinsic apoptotic pathways potentially are active in Bombyx mori. Conclusionsīombyx mori possesses potential apoptosis-related genes. Sequence analyses revealed that these genes could have key roles in apoptosis. Twenty-three of these apoptosis-related genes were cloned and sequenced using cDNA templates isolated from BmE-SWU1 cells. Moreover, we identified a new caspase family member, BmCaspase-New, two splice variants of BmDronc, and Bm3585, a mammalian TNF superfamily member homolog. Fifty-two apoptosis-related candidate genes were identified, including five caspase family members, two tumor necrosis factor (TNF) superfamily members, one Bcl-2 family member, four baculovirus IAP (inhibitor of apoptosis) repeat (BIR) domain family members and 1 RHG (Reaper, Hid, Grim, and Sickle Drosophila cell death activators) family member. Resultsįrom the newly assembled genome databases, a genome-wide analysis of apoptosis-related genes in Bombyx mori was performed using both nucleotide and protein Blast searches. Herein we have identified apoptosis-related genes in the silkworm Bombyx mori and compared them to those from insects, mammals, and nematodes. However, the lack of genomic data for silkworms limits their usefulness in apoptosis studies, despite the advantages of silkworm as a representative of Lepidoptera and an effective model system. ![]() Recently, substantial studies have been undertaken on apoptosis in model animals including humans, fruit flies, and the nematode. Apoptosis is regulated in an orderly fashion by a series of genes, and has a crucial role in important physiological processes such as growth development, immunological response and so on.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |